| First Author | Cronshaw JM | Year | 2002 |
| Journal | J Cell Biol | Volume | 158 |
| Issue | 5 | Pages | 915-27 |
| PubMed ID | 12196509 | Mgi Jnum | J:145212 |
| Mgi Id | MGI:3833823 | Doi | 10.1083/jcb.200206106 |
| Citation | Cronshaw JM, et al. (2002) Proteomic analysis of the mammalian nuclear pore complex. J Cell Biol 158(5):915-27 |
| abstractText | As the sole site of nucleocytoplasmic transport, the nuclear pore complex (NPC) has a vital cellular role. Nonetheless, much remains to be learned about many fundamental aspects of NPC function. To further understand the structure and function of the mammalian NPC, we have completed a proteomic analysis to identify and classify all of its protein components. We used mass spectrometry to identify all proteins present in a biochemically purified NPC fraction. Based on previous characterization, sequence homology, and subcellular localization, 29 of these proteins were classified as nucleoporins, and a further 18 were classified as NPC-associated proteins. Among the 29 nucleoporins were six previously undiscovered nucleoporins and a novel family of WD repeat nucleoporins. One of these WD repeat nucleoporins is ALADIN, the gene mutated in triple-A (or Allgrove) syndrome. Our analysis defines the proteome of the mammalian NPC for the first time and paves the way for a more detailed characterization of NPC structure and function. |
