| First Author | Rudnicka W | Year | 1997 |
| Journal | Microbiol Immunol | Volume | 41 |
| Issue | 11 | Pages | 847-53 |
| PubMed ID | 9444325 | Mgi Jnum | J:169995 |
| Mgi Id | MGI:4943671 | Doi | 10.1111/j.1348-0421.1997.tb01939.x |
| Citation | Rudnicka W, et al. (1997) The host response to Listeria monocytogenes mutants defective in genes encoding phospholipases C (plcA, plcB) and actin assembly (actA). Microbiol Immunol 41(11):847-53 |
| abstractText | Several genes involved in the determination of Listeria monocytogenes pathogenesis have been identified. Among them, plcA gene encodes phosphatidylinositol-specific phospholipase C (PI-PLC), plcB gene encodes a broad-range phospholipase C (PC-PLC), and actA encodes a protein contributing to actin assembly in infected cells. The interaction of L. monocytogenes wild type (LO 28) strain and two derivative mutants, plcA- (BUG 206) and actA-/plcB- (LUT 12), with macrophages and T lymphocytes was investigated in a mouse model of listeriosis. Both mutants showed evidence of attenuation. The plcA- mutant, but not the plcB- mutant, expressed an increase in susceptibility to the anti-listerial activity of macrophages. Both mutants showed a decreased ability to induce IL-12 production by bone marrow macrophages when co-stimulated with E. coli LPS or IFN-gamma. In vivo, L. monocytogenes plcA- mutant was found to be a more effective stimulator of T cells than the wild LO 28 strain. |
