| First Author | Gadhavi PL | Year | 2001 |
| Journal | Biochem Biophys Res Commun | Volume | 281 |
| Issue | 2 | Pages | 520-8 |
| PubMed ID | 11181078 | Mgi Jnum | J:67606 |
| Mgi Id | MGI:1930906 | Doi | 10.1006/bbrc.2001.4375 |
| Citation | Gadhavi PL, et al. (2001) The regulatory region of the human desmocollin 3 promoter forms a DNA four-way junction. Biochem Biophys Res Commun 281(2):520-8 |
| abstractText | Adhesion between desmosomal junctions is mediated by structural proteins of the cadherin family, viz. three desmocollins (DSC) and three desmogleins (DSG). Promoter and primer extension analysis of human DSC3 showed a TATA-less sequence initiating transcription via a cluster of sites upstream of the coding region. Deletion analysis of 1 kb of the promoter showed that expression is regulated between --303 and --203 bp upstream of the start-site of translation. Tertiary structure analysis of this cis-active region (cis 1) revealed a potential DNA 4-way junction which is notably G/C-rich in sequence. PAGE analysis of this region identified four differently migrating forms of the DNA. Structure-specific cleavage of the DNA with bacteriophage T7 endonuclease I showed the slowest migrating form to be either an extended/cruciform or stacked-X 4-way junction. DNA-binding, gel retardation assays of the cis 1 region showed distinct DNA-protein complexes and by competition experiments and using purified junction DNA we show that one of these complexes bound with both sequence and structure specificity to the 4-way junction DNA. Copyright 2001 Academic Press. |
