| First Author | Arjomand J | Year | 2001 |
| Journal | J Neurochem | Volume | 77 |
| Issue | 3 | Pages | 720-9 |
| PubMed ID | 11331401 | Mgi Jnum | J:69355 |
| Mgi Id | MGI:1934486 | Doi | 10.1046/j.1471-4159.2001.00219.x |
| Citation | Arjomand J, et al. (2001) Differential splicing of transcripts encoding the orphanin FQ/nociceptin precursor. J Neurochem 77(3):720-9 |
| abstractText | Orphanin FQ or nociceptin (OFQ/N), the heptadecapeptide agonist for the NOP receptor, is derived by proteolytic processing from a precursor protein, preproOFQ/N. Previous studies have reported alternative splicing between exons 3 and 4 of the mouse OFQ/N transcript, which, upon translation, would yield precursor proteins with different C-termini. Using RT-PCR, we identified similar alternative splicing of preproOFQ/N transcripts in humans and rats. In addition, we identified two novel human preproOFQ/N splice variants from which exon 2 has been excised and which also undergo alternative splicing between exons 3 and 4. Exon 2 contains the translational start site for preproOFQ/N and encodes the signal peptide sequence. In vitro translation of cRNAs lacking exon 2 yields shorter translation products which arise from an alternative initiator methionine located within exon 3. The resulting proteins would lack a signal peptide sequence, which would likely alter their cellular trafficking and processing. |
