| First Author | Catron T | Year | 2001 |
| Journal | Biochem Biophys Res Commun | Volume | 282 |
| Issue | 2 | Pages | 602-7 |
| PubMed ID | 11401503 | Mgi Jnum | J:68638 |
| Mgi Id | MGI:1933009 | Doi | 10.1006/bbrc.2001.4613 |
| Citation | Catron T, et al. (2001) Hypoxia Regulates Avian Cardiac Arnt and HIF-1alpha mRNA Expression. Biochem Biophys Res Commun 282(2):602-7 |
| abstractText | The aryl hydrocarbon receptor nuclear translocator (Arnt) and hypoxia-inducible factor (HIF)-1alpha mediate cellular responses to hypoxia. We investigated the ability of hypoxia to regulate Arnt and HIF-1alpha mRNA in the heart in vivo. We cloned avian Arnt, developed an in vivo model of chronic cardiac hypoxia, and measured expression of cardiac Arnt and HIF-1alpha mRNA by quantitative RT-PCR. Chronic hypoxic exposure (24 h to 15% O(2)) of day 9 chick embryos resulted in a 30-fold increase in covalent binding of (3)H-misonidazole, a hypoxic tissue marker, to cardiac tissue, and a 2-fold induction of cardiac inducible nitric oxide synthase mRNA, compared to normoxic controls. In this same model, cardiac Arnt mRNA expression decreased by 35%, while HIF-1alpha mRNA expression increased 400%. These data suggest that regulation of Arnt and HIF-1alpha mRNA expression may contribute to the physiological responses of the heart during prolonged hypoxia. Copyright 2001 Academic Press. |
