| First Author | Kojima T | Year | 2001 |
| Journal | Biochem Biophys Res Commun | Volume | 284 |
| Issue | 4 | Pages | 1039-47 |
| PubMed ID | 11409899 | Mgi Jnum | J:70024 |
| Mgi Id | MGI:2136089 | Doi | 10.1006/bbrc.2001.5080 |
| Citation | Kojima T, et al. (2001) Genomic organization of the Shc-related phosphotyrosine adapters and characterization of the full-length Sck/ShcB: specific association of p68-Sck/ShcB with pp135. Biochem Biophys Res Commun 284(4):1039-47 |
| abstractText | The Shc gene family is an emerging family, containing at least three members designated Shc/ShcA, Sck/Sli/ShcB, N-Shc/Rai/ShcC in mammals. In this study, we determined the genomic organization of the mouse Shc family. Coding regions of ShcA, B, and C each comprised 12 exons, spanned approximately 6, 20, and 65 kb, and located on chromosome 3, 10, and 13, respectively. Based on this genome analysis, we determined the full-length structure of mouse Sck/ShcB as a 68-kD protein. We found that the 68-kD full-length Sck/ShcB was more efficiently phosphorylated upon EGF treatment than the previously-analyzed CH2-deleted form. We also found that Sck specifically interacted with a 135-kD phosphoprotein (pp135) through its SH2 domain following membrane depolarization. The Sck-pp135 interaction was reduced by Src kinase inhibitors. These results suggest that Sck, but not N-Shc nor Shc, transmit signals in conjunction with pp135 following Src activation and/or calcium entry in the cell. Copyright 2001 Academic Press. |
