| First Author | Nakai Y | Year | 2001 |
| Journal | J Biol Chem | Volume | 276 |
| Issue | 11 | Pages | 8314-20 |
| PubMed ID | 11110795 | Mgi Jnum | J:68037 |
| Mgi Id | MGI:1931958 | Doi | 10.1074/jbc.M007878200 |
| Citation | Nakai Y, et al. (2001) Nuclear localization of yeast Nfs1p is required for cell survival. J Biol Chem 276(11):8314-20 |
| abstractText | Saccharomyces cerevisiae Nfs1p is mainly found in the mitochondrial matrix and has been shown to participate in iron-sulfur cluster assembly. We show here that Nfs1p contains a potential nuclear localization signal, RRRPR, in its mature part. When this sequence was mutated to RRGSR, the mutant protein could not restore cell growth under chromosomal NFS1-depleted conditions. However, this mutation did not affect the function of Nfs1p in biogenesis of mitochondrial iron-sulfur proteins. The growth defect of the mutant was complemented by simultaneous expression of the mature Nfs1p, which contains the intact nuclear localization signal but lacks its mitochondrial-targeting presequence. These results suggest that a fraction of Nfs1p is localized in the nucleus and is essential for cell viability. |
