| First Author | Nosaka K | Year | 2001 |
| Journal | Biochim Biophys Acta | Volume | 1517 |
| Issue | 2 | Pages | 293-7 |
| PubMed ID | 11342111 | Mgi Jnum | J:67315 |
| Mgi Id | MGI:1930378 | Doi | 10.1016/s0167-4781(00)00247-5 |
| Citation | Nosaka K, et al. (2001) Isolation and characterization of a human thiamine pyrophosphokinase cDNA. Biochim Biophys Acta 1517(2):293-7 |
| abstractText | A human thiamine pyrophosphokinase cDNA clone (hTPK1) was isolated and sequenced. When the intact hTPK1 open reading frame was expressed as a histidine-tag fusion protein in Escherichia coli, marked enzyme activity was detected in the bacterial cells. The hTPK1 mRNA was widely expressed in various human tissues at a very low level, and the mRNA content in cultured fibroblasts was unaffected by the thiamine concentration of the medium. The chromosome localization of the hTPK1 gene was assigned to 7q34. |
