| First Author | Ramjaun AR | Year | 2001 |
| Journal | J Biol Chem | Volume | 276 |
| Issue | 31 | Pages | 28913-9 |
| PubMed ID | 11384986 | Mgi Jnum | J:70713 |
| Mgi Id | MGI:2138032 | Doi | 10.1074/jbc.M103198200 |
| Citation | Ramjaun AR, et al. (2001) Endophilin regulates JNK activation through its interaction with the germinal center kinase-like kinase. J Biol Chem 276(31):28913-9 |
| abstractText | The endophilin family of proteins function in clathrin-mediated endocytosis. Here, we have identified and cloned the rat germinal center kinase-like kinase (rGLK), a member of the GCK (germinal center kinase) family of c-Jun N-terminal kinase (JNK) activating enzymes, as a novel endophilin I-binding partner. The interaction occurs both in vitro and in cells and is mediated by the Src homology 3 domain of endophilin I and a region of rGLK containing the endophilin consensus-binding sequence PPRPPPPR. Overlay analysis of rat brain extracts demonstrates that endophilin I is a major Src homology 3 domain-binding partner for rGLK. Overexpression of full-length endophilin I activates rGLK-mediated JNK activation, whereas N- and C-terminal fragments of endophilin I block JNK activation. Thus, endophilin I appears to have a novel function in JNK activation. |
