| First Author | Gonda H | Year | 2001 |
| Journal | Immunol Lett | Volume | 78 |
| Issue | 1 | Pages | 45-9 |
| PubMed ID | 11470151 | Mgi Jnum | J:70848 |
| Mgi Id | MGI:2148387 | Doi | 10.1016/s0165-2478(01)00232-2 |
| Citation | Gonda H, et al. (2001) DNA polymerase beta is not essential for the formation of palindromic (P) region of T cell receptor gene. Immunol Lett 78(1):45-9 |
| abstractText | Formation of palindromic (P) region at the variable (V)-diversity (D)-joining (J) junction in DNA polymerase beta (pol-beta) deficient mice were investigated by sequencing of reverse transcriptase-polymerase chain reaction (RT-PCR) products of mRNAs encoding the beta chain of T cell receptor (TCR). Total 42 and 43 cDNA clones encoding V(beta8)-D(beta)-J(beta)-C(beta) from E18.5 embryonic thymocytes of pol-beta gene knocked-out and wild type control mouse, respectively, were sequenced. Among them five and six clones from pol-beta knocked-out and wild type, respectively, have P insertions of two nucleotides. This result unequivocally indicates that pol-beta, which is one of the repair-type DNA polymerases most abundantly expressed in thymus and spleen, is not essential for the formation of P region. |
