| First Author | Hanna Z | Year | 2001 |
| Journal | Blood | Volume | 98 |
| Issue | 7 | Pages | 2275-8 |
| PubMed ID | 11568018 | Mgi Jnum | J:72110 |
| Mgi Id | MGI:2151738 | Doi | 10.1182/blood.v98.7.2275 |
| Citation | Hanna Z, et al. (2001) Distinct regulatory elements are required for faithful expression of human CD4 in T cells, macrophages, and dendritic cells of transgenic mice. Blood 98(7):2275-8 |
| abstractText | To identify the regulatory elements controlling expression of the human CD4 (hCD4) gene in different cell types of the immune system, deletion and chimeric (human/murine) reporter genes were constructed and tested in transgenic (Tg) mice. Regulatory elements required for the proper hCD4 expression in the immature double-positive thymic T cells were identified in the enhancer and in the 3' end of intron 1. Expression of hCD4 in macrophages is controlled by at least 2 sets of regulatory elements: one present in front of exon 1 and the second at the 5' end of intron 1. The hCD4 elements required for expression on both myeloid and lymphoid CD8alpha(+) dendritic cells (DCs) from lymph node and thymus were found to be different from those required for macrophage expression. The results indicate that expression of hCD4 in T cells, macrophages, and DCs is controlled by distinct regulatory elements. |
