| First Author | Kleines M | Year | 2001 |
| Journal | Gene | Volume | 275 |
| Issue | 1 | Pages | 157-62 |
| PubMed ID | 11574164 | Mgi Jnum | J:72038 |
| Mgi Id | MGI:2151657 | Doi | 10.1016/s0378-1119(01)00620-5 |
| Citation | Kleines M, et al. (2001) Cloning and expression of the human single copy homologue of the mouse zinc finger protein zfr. Gene 275(1):157-62 |
| abstractText | A human homologue of the murine zinc finger protein zfr is transcriptionally induced in the Epstein-Barr virus-positive Burkitt lymphoma cell line Raji upon treatment with the granulocyte/macrophage lineage ganglioside IV(3)NeuAc-nLcOse(4)Cer. The gene was cloned by a rapid amplification of cDNA ends approach based on a cDNA clone. The resulting hzfr sequence is 3393 base pairs in length coding for a protein of 1057 amino acids. Sequence alignments between hzfr and zfr reveal an identity of 92% on the nucleotide level and an identity of 96.4% on the amino acid level, respectively. Based on Southern blot data hzfr can be addressed as a single copy gene. Tissue-specific expression was determined by semi-quantitative PCR of normalized cDNA populations from various human tissues with glyceraldehyde-3-phosphate dehydrogenase as an internal control. Highest levels of transcripts were found in brain. hzfr transcripts could not be detected in skeletal muscle. |
