| First Author | Choi JD | Year | 2001 |
| Journal | Mamm Genome | Volume | 12 |
| Issue | 10 | Pages | 758-64 |
| PubMed ID | 11668390 | Mgi Jnum | J:72512 |
| Mgi Id | MGI:2153185 | Doi | 10.1007/s00335-001-3027-5 |
| Citation | Choi JD, et al. (2001) Microarray expression profiling of tissues from mice with uniparental duplications of Chromosomes 7 and 11 to identify imprinted genes. Mamm Genome 12(10):758-64 |
| abstractText | Microarray analysis allows the screening of thousands of identifiable genes in a single experiment. The challenge of this approach is to combine the new technology with established genetic tools to associate genes with specific biological function. In this study we have designed a screen to identify imprinted genes from mice with uniparental duplications of proximal Chromosomes (Chrs) 7 and 11, using microarray analysis. By comparing the expression patterns in embryonic and newborn tissues of maternally versus paternally inherited proximal Chrs 7 and 11, we have correctly identified four out of five known imprinted genes represented on a microarray. We have additionally identified two novel imprinted candidate genes as well as a differentially expressed clone that is a potential downstream target. Interpretation of the microarray data requires careful preparation of age- and strain-matched samples and attention to detail in tissue dissection technique. |
