| First Author | Iozef R | Year | 2000 |
| Journal | Biochim Biophys Acta | Volume | 1500 |
| Issue | 1 | Pages | 137-41 |
| PubMed ID | 10564726 | Mgi Jnum | J:58588 |
| Mgi Id | MGI:1349250 | Doi | 10.1016/s0925-4439(99)00094-0 |
| Citation | Iozef R, et al. (2000) Assembly and functional expression of murine glutathione reductase cDNA: a sequence missing in expressed sequence tag libraries. Biochim Biophys Acta 1500(1):137-41 |
| abstractText | Glutathione reductase (GR) is a chemotherapeutic target. Murine GRcDNA, which contains 85% GC in the 38 codons following the start codon, was assembled from the PCR-amplified exon 1 and a downstream cDNA prior to expression in Escherichia coli as a His(6)-tagged protein. Recombinant GR, an FAD-containing homodimer, corresponds in its enzymic and spectral properties to GR isolated from murine Ehrlich ascites tumor cells. Another cDNA, representing GR with a mitochondrial targeting sequence, yielded two distinct enzymically active expression products. |
