| First Author | Jørgensen MC | Year | 1999 |
| Journal | FEBS Lett | Volume | 461 |
| Issue | 3 | Pages | 287-94 |
| PubMed ID | 10567713 | Mgi Jnum | J:58644 |
| Mgi Id | MGI:1349306 | Doi | 10.1016/s0014-5793(99)01436-2 |
| Citation | Jorgensen MC, et al. (1999) Cloning and DNA-binding properties of the rat pancreatic beta-cell-specific factor Nkx6.1. FEBS Lett 461(3):287-94 |
| abstractText | The homeodomain (HD) protein Nkx6.1 is the most beta-cell-specific transcription factor known in the pancreas and its function is critical for the formation of the insulin-producing beta-cells. However, the target genes, DNA-binding site, and transcriptional properties of Nkx6.1 are unknown. Using in vitro binding site selection we have identified the DNA sequence of the Nkx6.1 binding site to be TTAATTG/A. A reporter plasmid containing four copies of this sequence is activated by an Nkx6.1HD/VP16 fusion construct. Full-length Nkx6.1 fails to activate this reporter plasmid in spite of robust interaction with the binding site in vitro. Stable expression of Nkx6.1 in the glucagon-producing alpha-cell-like MSL-G-AN cells induces expression of the endogenous insulin gene in a subset of the cell population. The expression of other known beta-cell-specific factors such as Pax4, Pax6, Pdx1, GLUT2 and GLP1-R is unchanged by the introduction of Nkx6.1. |
