| First Author | Sobue K | Year | 1999 |
| Journal | Biochim Biophys Acta | Volume | 1489 |
| Issue | 2-3 | Pages | 393-8 |
| PubMed ID | 10673041 | Mgi Jnum | J:59138 |
| Mgi Id | MGI:1351073 | Doi | 10.1016/s0167-4781(99)00194-3 |
| Citation | Sobue K, et al. (1999) Molecular cloning of two bovine aquaporin-4 cDNA isoforms and their expression in brain endothelial cells. Biochim Biophys Acta 1489(2-3):393-8 |
| abstractText | Two cDNA isoforms of bovine aquaporin-4 (bAQP4-A and bAQP4-B) were newly isolated. Sequence analysis of both cDNAs revealed open reading frames of 972 (bAQP4-A) and 906 nucleotides (bAQP4-B) with deduced proteins of 323 (bAQP4-A) and 301 amino acid residues (bAQP4-B). Partial 5'-genomic sequence analysis showed that the 5'-noncoding sequences specific to bAQP4-A and -B transcripts were contained in distinct exons, exon 0 for bAQP4-A and new exon X for bAQP4-B. RNase protection assay demonstrated the definite expression of both isoforms in bovine brain. The deduced amino acid sequence of bAQP4-A was highly homologous to the human (97%), rat (95%), and mouse (93%) AQP4. Reverse transcription-PCR detected the expression of AQP4 mRNAs in bovine brain endothelial cells as well as in a variety of bovine organs such as brain, lung, spleen, and kidney. Northern blot analysis indicated that a 6.0 kb message is predominantly expressed in bovine brain and lung. |
