| First Author | Magoulas C | Year | 2000 |
| Journal | Gene | Volume | 243 |
| Issue | 1-2 | Pages | 115-23 |
| PubMed ID | 10675619 | Mgi Jnum | J:60372 |
| Mgi Id | MGI:1353218 | Doi | 10.1016/s0378-1119(99)00551-x |
| Citation | Magoulas C, et al. (2000) Isolation and genomic analysis of the human surf-6 gene: a member of the Surfeit locus. Gene 243(1-2):115-23 |
| abstractText | The human Surfeit locus contains at least six tightly clustered genes (Surf-1 to Surf-6) of which five (Surf-1 to Surf-5) have been characterised and found not to share any sequence homology. The organisation and juxtaposition of the Surfeit genes are conserved between human and mouse. The Surf-6 gene that encodes a novel nucleolar-matrix protein with nucleic-acid binding properties has been characterised in mouse. In this work, we have isolated and analysed the human Surf-6 homologue and determined its genomic organisation in the Surfeit locus. The human Surf-6 gene has five exons spread over a distance of 4.3kb and has features of a housekeeping gene being ubiquitously expressed, having its 5' end located within a CpG rich island and lacking a canonical TATA box. The intragenic region between the 3' end of the Surf-5 gene and the 5' end of the Surf-6 gene is 3.2kb and contains a pseudogene of the ribosomal protein gene rpL21. The putative human Surf-6 protein is 361 amino acids long and includes motifs found in both the mouse and fish Surf-6 homologues, which may underlie the functions of Surf-6. Three amino acid polymorphisms have been detected at codons 163, 175 and 311 by SSCP analysis. |
