| First Author | Murray RC | Year | 2000 |
| Journal | Dev Dyn | Volume | 218 |
| Issue | 1 | Pages | 189-94 |
| PubMed ID | 10822270 | Mgi Jnum | J:62071 |
| Mgi Id | MGI:1858298 | Doi | 10.1002/(SICI)1097-0177(200005)218:1<189::AID-DVDY16>3.0.CO;2-4 |
| Citation | Murray RC, et al. (2000) A fragment of the Neurogenin1 gene confers regulated expression of a reporter gene in vitro and in vivo. Dev Dyn 218(1):189-94 |
| abstractText | The basic helix-loop-helix transcription factor neurogenin1 is required for proper nervous system development in vertebrates. It is expressed in neuronal precursors during embryonic development and is thought to play a role in specifying neuronal fate. To investigate the regulation of neurogenin1 expression, the transcriptional start site of the gene was identified and a 2.7-kb fragment ending in the first exon was shown to possess basal promoter activity. This 2.7-kb fragment was able to promote expression of reporter genes in P19 cells under conditions in which expression of endogenous neurogenin1 was induced. Importantly, the 2.7-kb fragment was able to drive expression of a lacZ reporter gene in transgenic mice in most tissues in which neurogenin1 is normally expressed, including those peripheral ganglia that fail to develop in neurogenin1 'knockout' mice. These findings identify a regulatory region containing elements responsible for appropriate expression of a gene with a crucial role in generating the vertebrate nervous system. |
