First Author | Denovan-Wright EM | Year | 2000 |
Journal | Biochim Biophys Acta | Volume | 1492 |
Issue | 1 | Pages | 221-6 |
PubMed ID | 11004493 | Mgi Jnum | J:62842 |
Mgi Id | MGI:1859759 | Doi | 10.1016/s0167-4781(00)00075-0 |
Citation | Denovan-Wright EM, et al. (2000) Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebrafish (Danio rerio)(1). Biochim Biophys Acta 1492(1):221-6 |
abstractText | We have determined the nucleotide sequence for two cDNA clones coding for a fatty acid binding protein (FABP) from zebrafish (Danio rerio). Comparison of the sequence with GenBank entries revealed extensive amino acid identity between this zebrafish FABP and brain FABPs (B-FABP) from other species. The zebrafish B-FABP cDNA hybridized to single restriction fragments of total zebrafish genomic DNA digested with the restriction endonucleases BglII or EcoRI suggesting that a single copy of the B-FABP gene is present in the zebrafish genome. Northern blot analysis demonstrated that the zebrafish B-FABP mRNA is approximately 850 nucleotides in length. In situ hybridization revealed that the B-FABP mRNA was expressed in the periventricular gray zone of the optic tectum of the adult zebrafish brain. |