| First Author | García-Frigola C | Year | 2000 |
| Journal | Neuroreport | Volume | 11 |
| Issue | 14 | Pages | 3181-5 |
| PubMed ID | 11043545 | Mgi Jnum | J:65338 |
| Mgi Id | MGI:1926390 | Doi | 10.1097/00001756-200009280-00027 |
| Citation | Garcia-Frigola C, et al. (2000) Mouse Tspan-5, a member of the tetraspanin superfamily, is highly expressed in brain cortical structures. Neuroreport 11(14):3181-5 |
| abstractText | Using a subtractive hybridization method for the identification of genes related to the development of the murine cerebral cortex, we cloned a mouse homologue of a human tetraspanin family member, Tspan-5. We have isolated a 3.1 Kb cDNA fragment containing the entire coding region. Analysis of the cDNA nucleotide sequence revealed that mouse Tspan-5 shares 98% amino acid sequence identity with its human homologue. The predicted length of the mouse protein is 268 amino acids, with four putative hydrophobic domains with N- and C-intracellular tails, and two extracellular domains. Northern blot analysis of adult mouse tissues showed a single transcript, which is preferentially expressed in the brain. In situ hybridization showed prominent expression of Tspan-5 in the neocortex, the hippocampus, amygdala and in Purkinje cells in the cerebellum. The pattern of expression of Tspan-5 in the mouse brain suggests a role for the tetraspanins in the maintenance of adult brain function. |
