| First Author | Zhuang M | Year | 2000 |
| Journal | Brain Res Mol Brain Res | Volume | 82 |
| Issue | 1-2 | Pages | 52-64 |
| PubMed ID | 11042357 | Mgi Jnum | J:65446 |
| Mgi Id | MGI:1926586 | Doi | 10.1016/s0169-328x(00)00177-7 |
| Citation | Zhuang M, et al. (2000) Differential regulation of two period genes in the xenopus eye. Brain Res Mol Brain Res 82(1-2):52-64 |
| abstractText | The recent identification and analysis of mammalian homologues of the well characterized Drosophila circadian clock gene, Period (Per), has led to the idea that key features of vertebrate circadian rhythmicity are conserved at the molecular level. The Xenopus laevis retina contains a circadian clock mechanism that can be studied in vitro. To study the rhythmic expression of Per in the Xenopus retina, we used a degenerate RT-PCR strategy to obtain cDNA clones covering the entire 1427 amino acid coding region of a Xenopus homologue of Per2 and a partial cDNA sequence for a Xenopus homologue of Per1. Northern blot analysis shows that xPer1 and xPer2 transcripts are expressed most abundantly in the eye and the brain. However, rhythmic expression of xPer2 transcripts in the retina and retinal pigment epithelium (RPE) is light dependent and occurs only under 12 h light/12 h dark (LD) conditions, not in constant dark (DD). In contrast, xPer1 mRNA accumulation is rhythmic under both LD and DD conditions. Light dependent regulation of xPer2 mRNA and circadian regulation of xPer1 mRNA in the Xenopus retina differs from that in Drosophila and mammals. Light dependence of xPer2 mRNA levels and the offset phase relationship of the xPer2 rhythm to that for xPer1 suggests a role for xPer2 in circadian entrainment. |
