| First Author | Toyosawa S | Year | 2000 |
| Journal | Gene | Volume | 256 |
| Issue | 1-2 | Pages | 1-11 |
| PubMed ID | 11054529 | Mgi Jnum | J:65493 |
| Mgi Id | MGI:1926664 | Doi | 10.1016/s0378-1119(00)00379-6 |
| Citation | Toyosawa S, et al. (2000) Cloning and characterization of the human ameloblastin gene. Gene 256(1-2):1-11 |
| abstractText | We isolated the full-length human ameloblastin (AMBN) cDNA clone using reverse transcription-polymerase chain reaction (RT-PCR) methods. Sequence analysis of the AMBN cDNA revealed an open reading frame of 1341bp encoding a 447-amino-acid protein. Comparison with pig, cattle, rat, and mouse AMBN sequences showed a high amino acid sequence similarity and led to the identification of a novel 78bp (26 amino acids) insert resulting from internal sequence duplication. By DNA analysis of a human genomic clones, the AMBN gene was shown to consist of 13 exons and a novel 78bp segment, which proved to comprise two small exons. Human ameloblastomas express AMBN transcripts that contain some mutations. |
