| First Author | Sloop KW | Year | 2000 |
| Journal | Mol Cell Endocrinol | Volume | 168 |
| Issue | 1-2 | Pages | 77-87 |
| PubMed ID | 11064154 | Mgi Jnum | J:65578 |
| Mgi Id | MGI:1926756 | Doi | 10.1016/s0303-7207(00)00318-x |
| Citation | Sloop KW, et al. (2000) Biochemical and genetic characterization of the porcine prophet of pit-1 pituitary transcription factor. Mol Cell Endocrinol 168(1-2):77-87 |
| abstractText | Prophet of Pit-1 (Prop-1) is a paired class homeodomain transcription factor that is specifically expressed in the pituitary gland. Mutations in the Prop-1 gene cause compound pituitary diseases in mouse models and human patients. We have cloned and analyzed the porcine ortholog of Prop-1. Analysis of cDNAs revealed that the porcine Prop-1 sequence is similar to the mouse and human proteins within the homeodomain and carboxyl terminus, but the amino terminus is poorly conserved. The Prop-1 gene consists of three exons and two introns and spans 3.8 kilobases of genomic DNA. In addition, we mapped Prop-1 to the q arm of pig chromosome two. During development, Prop-1 is expressed at the time of pituitary organogenesis. In the adult, expression was observed at low levels only in the pituitary gland. The porcine Prop-1 protein displays similar biochemical, DNA binding, and transcriptional activities to human PROP-1. We conclude that, although the structural divergence between the porcine and human PROP-1 molecules may indicate some distinct functions, the porcine Prop-1 gene encodes a pituitary transcription factor with similar overall activities to the human ortholog. |
