| First Author | Im DS | Year | 2000 |
| Journal | Biochem Biophys Res Commun | Volume | 279 |
| Issue | 1 | Pages | 139-43 |
| PubMed ID | 11112429 | Mgi Jnum | J:66289 |
| Mgi Id | MGI:1928233 | Doi | 10.1006/bbrc.2000.3933 |
| Citation | Im DS, et al. (2000) Characterization of a zebrafish (Danio rerio) sphingosine 1-phosphate receptor expressed in the embryonic brain. Biochem Biophys Res Commun 279(1):139-43 |
| abstractText | Sphingosine 1-phosphate elicits a variety of responses in mammals via at least five G protein-coupled Edg receptors. We cloned zebrafish edg1 and expressed it in Rh7777 cells. In these cultures, S1P inhibited forskolin-driven rises in cAMP and this response was eliminated by pretreatment of the cultures with pertussis toxin. In Rh7777 membranes, S1P stimulated GTPgamma[(35)S] binding 2-3 fold. Zebrafish edg1 is expressed in embryonic brain, particularly ventral diencephalon, optic stalks, and anterior hindbrain. Our findings suggest that nonmammalian vertebrates use S1P to signal during embryogenesis and that the properties of Edg1 receptor have been conserved for 400 million years. Copyright 2000 Academic Press. |
