| First Author | Lee CY | Year | 1979 |
| Journal | Biochem Genet | Volume | 17 |
| Issue | 7-8 | Pages | 631-4 |
| PubMed ID | 94263 | Mgi Jnum | J:6288 |
| Mgi Id | MGI:54765 | Doi | 10.1007/BF00502123 |
| Citation | Lee CY, et al. (1979) Biochemical and immunological studies of three genetic variants of 3-phosphoglycerate kinase 2 from the mouse. Biochem Genet 17(7-8):631-4 |
| abstractText | Three electrophoretic variants of 3-phosphoglycerate kinase 2 (PGK-2A,PGK-2B, and PGK-2C) were purified from DBA/2J, C3H/HeJ, and C57L/J mice, respectively. PGK-2C exhibits only 2% of the specific activity of PGK-2A and PGK-2B in the reaction leading to the formation of 1,3-diphosphoglycerate. Compared to PGK-2A and PGK-2B, PGK-2C exhibits broader coenzyme specificity and lower Kms for substrate and coenzymes. Incubation at 45C revealed immunionactivation and double immunodiffusion studies showed that mice carrying any one of these three PGK-2 alleles have similar amounts of proteins for PGK-1 and PGK-2 in testes. The results of these studies suggest that low PGK-2C activity in C57L/J mice is a result of a structural rather than a regulatory gene mutation. |
