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Publication : Molecular weight determination of two genetically linked cell surface murine antigens: ThB and Ly-6.

First Author  Matossian-Rogers A Year  1982
Journal  Immunogenetics Volume  15
Issue  6 Pages  591-9
PubMed ID  7106866 Mgi Jnum  J:6829
Mgi Id  MGI:55301 Doi  10.1007/BF00347053
Citation  Matossian-Rogers A, et al. (1982) Molecular weight determination of two genetically linked cell surface murine antigens: ThB and Ly-6. Immunogenetics 15(6):591-9
abstractText  Various murine tumor lines were screened by FACS analysis for the surface antigens ThB and Ly-6.2. Positive cell lines were used for immunoprecipitation studies. A monoclonal ThB-specific antibody immunoprecipitated a unique acidic protein of approximately 16 000 daltons from several positive tumors and from concanavalin A (Con-A) and LPS activated splenic lymphocytes. Monoclonal Ly-6.2-specific antibody was used to immunoprecipitate a 33 500 dalton protein that was shown to exist in four similarly sized forms with different basic charges. In the course of these studies, the apparent molecular weight of the surface antigen T 30, immunoprecipitated with a monoclonal T 30-specific antibody from the cell line EL4, was found to be approximately 25 000 daltons.
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