| First Author | Lewis S | Year | 1982 |
| Journal | Cell | Volume | 30 |
| Issue | 3 | Pages | 807-16 |
| PubMed ID | 6291785 | Mgi Jnum | J:6897 |
| Mgi Id | MGI:55369 | Doi | 10.1016/0092-8674(82)90285-9 |
| Citation | Lewis S, et al. (1982) Continuing kappa-gene rearrangement in a cell line transformed by Abelson murine leukemia virus. Cell 30(3):807-16 |
| abstractText | A cell line transformed by Abelson murine leukemia virus, called PD, is capable of carrying out kappa-gene rearrangement while growing in culture. Subclones of PD have diverse kappa-gene structures, and some derivatives show evidence of continued joining activity after as many as three subclonings. Analysis of PD sublineages has shown that a rearranged chromosome can undergo secondary kappa-gene rearrangements, producing either a new rearrangement or a deletion of C kappa. Although the PD line actively rearranges its kappa genes, its rearranged heavy-chain genes show little variation, and there is no rearrangement of lambda genes. In PD subclones, DNA fragments representing the reciprocal product of kappa-gene rearrangement are often evident, and they may undergo either further rearrangement or deletion. The implications of multiple rearrangements on a single chromosome and of the maintenance of reciprocal fragments are considered in the context of a model that postulates that the V kappa and J kappa segments are not all organized in the DNA in the same transcriptional direction, leading to inversions rather than deletions during joining. |
