| First Author | Curran T | Year | 1982 |
| Journal | J Virol | Volume | 44 |
| Issue | 2 | Pages | 674-82 |
| PubMed ID | 6292525 | Mgi Jnum | J:6904 |
| Mgi Id | MGI:55376 | Doi | 10.1128/jvi.44.2.674-682.1982 |
| Citation | Curran T, et al. (1982) FBJ murine osteosarcoma virus: identification and molecular cloning of biologically active proviral DNA. J Virol 44(2):674-82 |
| abstractText | A 12.0-kilobase EcoRI restriction fragment containing FBJ murine osteosarcoma virus (FBJ-MSV) proviral DNA was identified in FBJ-MSV-transformed nonproducer rat cells and molecularly cloned in bacteriophage Charon 30 (lambda FBJ-1). A 5.8-kb HindIII fragment containing the entire FBJ-MSV proviral DNA was isolated from lambda FBJ-1 and subsequently subcloned in plasmid pBR322 (pFBJ-2). The DNA from recombinant plasmid pFBJ-2 was able to induce morphological transformation of rat fibroblasts in tissue culture. Transfected cells contained the p55 and p39 antigens specific for cells transformed by FBJ-MSV (T. Curran and N. M. Teich, J. Virol. 42:114-122, 1982). The organization of the FBJ-MSV provirus was analyzed by restriction endonuclease mapping, and a region of nonhomology with the helper virus was delineated. Sequences specific for this region (presumably the viral fos gene) were subcloned and used as a probe to identify related sequences present in the normal genomes of cells from a variety of mammalian species (cellular fos). A single-size (3.4 kilobases long) class of RNA hybridizing to the viral fos probe was identified in FBJ-MSV-transformed cells. |
