| First Author | Symington FW | Year | 1982 |
| Journal | Immunogenetics | Volume | 16 |
| Issue | 5 | Pages | 381-91 |
| PubMed ID | 6984010 | Mgi Jnum | J:6945 |
| Mgi Id | MGI:55416 | Doi | 10.1007/BF00372098 |
| Citation | Symington FW, et al. (1982) Lyb-8.2: A new B cell antigen defined and characterized with a monoclonal antibody. Immunogenetics 16(5):381-91 |
| abstractText | A DBA/1 B10.D2-specific monoclonal antibody (CY34) is described which defines a new murine B lymphocyte differentiation antigen designated Lyb-8.2. The ontogeny, strain distribution, and cell-surface density of the antigen were studied by radioimmunoassay and by fluorescence-activated cell sorter (FACS) analysis. Lyb-8.2 appears to be expressed on pre-B cells and on all mature B lymphocytes. Lyb-8.2 molecules immunoprecipitated from surface labeled B10.D2 spleen cells migrated in polyacrylamide gels with an apparent mol. wt. of 95,000-105,000 daltons and were bound by lentil lectin. The expression of Lyb-8.2 is controlled by a locus on chromosome 7 that is closely linked to Gpi-1 and RP-2. Added Lyb-8.2-specific antibody did not measurably impair B lymphocyte function in several in vitro systems studied. |
