| First Author | Fournier RE | Year | 1983 |
| Journal | Somatic Cell Genet | Volume | 9 |
| Issue | 1 | Pages | 69-84 |
| PubMed ID | 6687641 | Mgi Jnum | J:7037 |
| Mgi Id | MGI:55508 | Doi | 10.1007/BF01544049 |
| Citation | Fournier RE, et al. (1983) Complementation mapping in microcell hybrids: localization of Fpgs and Ak-1 on Mus musculus chromosome 2. Somatic Cell Genet 9(1):69-84 |
| abstractText | The gene encoding folylpolyglutamyl synthetase (FPGS) was assigned to mouse chromosome 2 by complementation mapping. Chinese hamster ovary cells (AuxBl) deficient in FPGS, and consequently auxotrophic for glycine, adenosine, and thymidine (gat-), were employed as recipients in microcell-mediated chromosome transfer experiments. Mouse chromosomes derived from diploid embryo fibroblasts were introduced into hamster AuxBl cells, and gat+ microcell hybrids were selected in medium lacking adenosine and thymidine. Mouse chromosome 2 was the only donor chromosome whose presence correlated with expression of FPGS activity. Furthermore, every gat+ hybrid clone expressed murine AK-1, a marker previously assigned to chromosome 2. Eight of 20 clones analyzed retained deletion chromosomes derived from mouse chromosome 2. These clones were used to localize murine Fpgs and Ak-1 to a region of this chromosome, namely 2 (cen leads to Cl). |
