| First Author | Röhme D | Year | 1984 |
| Journal | Cell | Volume | 36 |
| Issue | 3 | Pages | 783-8 |
| PubMed ID | 6697397 | Mgi Jnum | J:7328 |
| Mgi Id | MGI:55799 | Doi | 10.1016/0092-8674(84)90358-1 |
| Citation | Rohme D, et al. (1984) Molecular clones of the mouse t complex derived from microdissected metaphase chromosomes. Cell 36(3):783-8 |
| abstractText | Fragments of the proximal half of mouse chromosome 17 including the t-complex region were microdissected from metaphase spreads. DNA was isolated from a pool of such fragments, and was cloned on microscale. Individual clones were used to probe genomic digests of DNA from a pair of Chinese hamster cell lines with or without mouse chromosome 17, and livers of congenic inbred lines of mice carrying wild-type and/or t-haplotype forms of chromosome 17. The data obtained indicate that 95% of the low copy number microclone inserts recognize DNA sequences present on mouse chromosome 17. It has been possible to use one-third of these clones to identify restriction-fragment-length polymorphisms between wild-type and t-haplotype DNA on a congenic background. These results demonstrate that these clones have been derived from the t-complex or regions closely linked to it. Clones of this type should provide starting points for a molecular analysis of this region of the mouse genome. |
