| First Author | Xin JH | Year | 1982 |
| Journal | EMBO J | Volume | 1 |
| Issue | 4 | Pages | 467-71 |
| PubMed ID | 6329684 | Mgi Jnum | J:32384 |
| Mgi Id | MGI:79881 | Doi | 10.1002/j.1460-2075.1982.tb01192.x |
| Citation | Xin JH, et al. (1982) Identification of an H-2Kd gene using a specific cDNA probe. EMBO J 1(4):467-71 |
| abstractText | A cDNA clone known to code for a mouse histocompatibility (class I) antigen was found to contain a sequence specific for a subpopulation of H-2 genes. This unique sequence is located in the 3' non-coding region close to the stretch of poly(A) nucleotides. A subclone containing this fragment (pH-2d-5) has been used to select hybridizing mRNA. Translation of the mRNA in vitro shows that H-2Kd mRNA is selected. Southern blot analysis of DNA from congenic recombinant mice show that at least one gene containing this sequence is located at the K locus (region) of the major histocompatibility complex. This gene contains a 3.7-kb BglII and a 13-kb EcoRI restriction endonuclease fragment. This gene has been isolated from a genomic DNA library. |
