| First Author | Alonso A | Year | 1985 |
| Journal | J Immunol | Volume | 135 |
| Issue | 1 | Pages | 614-9 |
| PubMed ID | 2987352 | Mgi Jnum | J:20058 |
| Mgi Id | MGI:68171 | Doi | 10.4049/jimmunol.135.1.614 |
| Citation | Alonso A, et al. (1985) Mouse immunoglobulin gene rearrangements. The sequence of a nonexpressed lambda 3-chain gene. J Immunol 135(1):614-9 |
| abstractText | A functionally defective lambda 3-immunoglobulin chain gene has been cloned from plasmacytoma HOPC-1 (gamma 2b, lambda 1). The lambda 3 gene resulted from the juxtaposition of the germline V lambda 1 sequence with a J lambda 3 C lambda 3 gene segment. DNA sequencing of the rearranged V lambda 1 J lambda 3 exon showed the presence of a single base pair deletion at the site of V-J joining. The alteration in the reading frame caused by this deletion generated a stop codon at the 3' end of J lambda 3, thus rendering this gene nonfunctional for light chain production. In addition, a one-point mutation in the J lambda 3-C lambda 3 intron distinguishes the rearranged gene from the unrearranged counterpart. The implications that this rearrangement has in terms of the mechanism of somatic mutations and of selective proliferation of B cells mediated by antigen stimulation are discussed. |
