| First Author | Melber K | Year | 1986 |
| Journal | Carcinogenesis | Volume | 7 |
| Issue | 2 | Pages | 317-22 |
| PubMed ID | 3004776 | Mgi Jnum | J:22457 |
| Mgi Id | MGI:70327 | Doi | 10.1093/carcin/7.2.317 |
| Citation | Melber K, et al. (1986) Molecular cloning of sequences activated during multi-stage carcinogenesis in mouse skin. Carcinogenesis 7(2):317-22 |
| abstractText | Recombinant DNA techniques were employed to isolate sequences that were activated during multi-stage carcinogenesis in the skin of NMRI mice. Differential screening of 5000 cDNA clones made from poly(A)+ RNA of squamous cell carcinomas induced by 7,12-dimethylbenz[a]anthracene (DMBA) and the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) resulted in the identification of 35 cDNA clones displaying a different hybridization signal. Eight cDNA clones, three of which proved to be identical, were used as probes in transfer hybridization experiments. These cDNA clones, designated pmal-1 to pmal-6, showed a strong signal with carcinoma RNA but either a weak or no signal with RNA from normal epidermis. Clone pmal-2 contained repetitive sequences as shown by Southern analysis resulting in a smeared RNA hybridization signal in RNA blots whereas the other five clones corresponded to discrete size classes of mRNA. Studies on the expression pattern of mal-1,-2 and -3 related sequences revealed transcriptional activation already in the benign papilloma stage of multi-step carcinogenesis. |
