| First Author | Croyle ML | Year | 1986 |
| Journal | DNA | Volume | 5 |
| Issue | 4 | Pages | 299-304 |
| PubMed ID | 3017658 | Mgi Jnum | J:37393 |
| Mgi Id | MGI:84787 | Doi | 10.1089/dna.1986.5.299 |
| Citation | Croyle ML, et al. (1986) Analysis of the organization and nucleotide sequence of the chromosomal gene for the beta-subunit of rat thyrotropin. DNA 5(4):299-304 |
| abstractText | The gene for the beta-subunit of rat thyrotropin has been isolated from a library of rat DNA fragments cloned in bacteriophage lambda. The complete nucleotide sequence of the gene has been determined including a portion of 5'- and 3'-flanking regions. The rat TSH-beta gene contains approximately 4879 nucleotides which ultimately lead to the production of a mRNA of about 554 nucleotides, exclusive of the 3' poly(A) tract. The first exon which represents only 5' untranslated sequences of the mRNA, is separated from the second exon by a very large, 3.9-kbp intervening sequence. The second and third exons are separated by a small, 377-bp intervening sequence. Southern blot analysis of total genomic DNA demonstrated that the rat genome contains sequences similar to the cloned gene, suggesting that no rearrangements occurred during the cloning process. |
