| First Author | Uematsu Y | Year | 1986 |
| Journal | EMBO J | Volume | 5 |
| Issue | 9 | Pages | 2123-9 |
| PubMed ID | 3023057 | Mgi Jnum | J:8477 |
| Mgi Id | MGI:56943 | Doi | 10.1002/j.1460-2075.1986.tb04475.x |
| Citation | Uematsu Y, et al. (1986) Molecular characterization of a meiotic recombinational hotspot enhancing homologous equal crossing-over [published erratum appears in EMBO J 1986 Dec 1;5(12):3408]. EMBO J 5(9):2123-9 |
| abstractText | We have cloned and sequenced a meiotic recombinational hotspot between the A beta 3 and A beta 2 genes in the major histocompatibility complex (MHC) of the mouse. This recombinational hotspot in the Mus musculus castaneus cas3 haplotype was previously localized to a region of 9.5 kb of DNA in which five independent crossing-over events occurred at the unusually high frequency of 0.6%. Aside from cas3, the hotspot appears to be absent in many other MHC haplotypes. We have now confined the five recombinational breakpoints to a stretch of 3.5 kb of DNA. From the nucleotide sequence around the recombinational breakpoints, determined in the parental cas3 and b haplotypes as well as for two recombinant haplotypes, we show that the two recombinant haplotypes were generated by homologous equal crossing-over and place the breakpoints within two non-overlapping stretches of 10 and 36 bp, respectively. Comparison of the DNA sequences of the hotspot-positive cas3 and the hotspot-negative b haplotypes reveals a number of differences, in particular, a CAGA-repeat sequence which is present in CAS3 in six, but only four copies in C57BL/6 DNA. This repeat sequence is reminiscent of one in a previously characterized hotspot in the E beta gene. |
