| First Author | Darlington GJ | Year | 1986 |
| Journal | Mol Cell Biol | Volume | 6 |
| Issue | 4 | Pages | 969-75 |
| PubMed ID | 2431276 | Mgi Jnum | J:28230 |
| Mgi Id | MGI:75854 | Doi | 10.1128/mcb.6.4.969 |
| Citation | Darlington GJ, et al. (1986) Simultaneous expression of salivary and pancreatic amylase genes in cultured mouse hepatoma cells. Mol Cell Biol 6(4):969-75 |
| abstractText | The tissue-specific expression of two types of mouse amylase genes does not overlap in vivo; the Amy-1 locus is transcribed in the parotid gland and the liver, while expression of Amy-2 is limited to the pancreas. We identified a mouse hepatoma cell line, Hepa 1-6, in which both amylase genes can be simultaneously expressed. Amy-1 is constitutively active in these cells and is inducible by dexamethasone at the level of mRNA. We demonstrated that the liver-specific promoter of Amy-1 is utilized by the dexamethasone-treated hepatoma cells, and that glucocorticoid consensus sequences are present upstream of this promoter. Amy-2 is not detectable constitutively, but can be activated if the cells are cultured in serum-free medium containing dexamethasone. Expression of Amy-2 in a nonpancreatic cell type has not previously been observed. We speculate that induction of Amy-1 and activation of Amy-2 may involve different regulatory mechanisms. Hepa 1-6 cells provide an experimental system for molecular analysis of these events. |
