| First Author | Kalb VF | Year | 1986 |
| Journal | Gene | Volume | 45 |
| Issue | 3 | Pages | 237-45 |
| PubMed ID | 3542713 | Mgi Jnum | J:35013 |
| Mgi Id | MGI:82465 | Doi | 10.1016/0378-1119(86)90021-1 |
| Citation | Kalb VF, et al. (1986) Isolation of a cytochrome P-450 structural gene from Saccharomyces cerevisiae. Gene 45(3):237-45 |
| abstractText | We have transformed a Saccharomyces cerevisiae host with an S. cerevisiae genomic library contained in the shuttle vector YEp24 and screened the resultant transformants for resistance to ketoconazole (Kc), an inhibitor of the cytochrome P-450 (P-450) enzyme lanosterol 14 alpha-demethylase. Two plasmids were isolated which transformed yeast to both increased resistance to Kc and increased levels of total P-450. Hybrid-selection and immunoprecipitation experiments showed that these plasmids, pVK1 and pVK2, contained the structural gene for an S. cerevisiae P-450. This conclusion was confirmed by the nucleotide sequence of a portion of pVK2, which revealed an open reading frame encoding a characteristic P-450 heme-binding region. |
