| First Author | Oberbäumer I | Year | 1986 |
| Journal | Gene | Volume | 49 |
| Issue | 1 | Pages | 81-91 |
| PubMed ID | 2436975 | Mgi Jnum | J:14723 |
| Mgi Id | MGI:62886 | Doi | 10.1016/0378-1119(86)90387-2 |
| Citation | Oberbaumer I (1986) New pUC-derived expression vectors for rapid construction of cDNA libraries. Gene 49(1):81-91 |
| abstractText | We have constructed a new series of the pUC-derived plasmids with an extended polylinker obtained from M13tg131 [Kieny et al., Gene 26 (1983) 91-99]. These vectors allowed us to design a simplified version of the method of Okayama and Berg [Mol. Cell. Biol. 2 (1982) 161-170] for establishing complete cDNA libraries. Improvements included easy recovery of the inserted cDNA due to the extended polylinkers; use of these vectors for gene expression in Escherichia coli, and amenability to supercoil sequencing with the universal primers of the M13 system [Chen and Seeburg, DNA 4 (1985) 165-170], which speeds up the identification of positive clones. Moreover, there is no need for an additional linker fragment, as was required by the Okayama and Berg [Mol. Cell. Biol. 2 (1982) 161-170] method. We have successfully used this system to obtain cDNA clones coding for the different chains of the large basement membrane proteins type IV collagen and laminin. |
