| First Author | Kasahara M | Year | 1987 |
| Journal | Proc Natl Acad Sci U S A | Volume | 84 |
| Issue | 10 | Pages | 3325-8 |
| PubMed ID | 3472212 | Mgi Jnum | J:8695 |
| Mgi Id | MGI:57160 | Doi | 10.1073/pnas.84.10.3325 |
| Citation | Kasahara M, et al. (1987) Random cloning of genes from mouse chromosome 17. Proc Natl Acad Sci U S A 84(10):3325-8 |
| abstractText | We describe a method for isolating cosmid clones randomly from mouse chromosome 17. A cosmid library was constructed from the mouse-Chinese hamster cell line R4 4-1 that contains a limited amount of mouse DNA (chromosomes 17 and 18 and some other unidentified material) on a Chinese hamster background. The library was screened with the murine repetitive sequence probe pMBA14, which selectively hybridizes with mouse DNA. The mouse-derived cosmid clones thus identified were individually hybridized with DNA from the mouse-Syrian hamster cell line JS17 containing all mouse chromosomes except chromosome 17 on a Syrian hamster background. We deduced that the cosmid clones that contained sequences absent in JS17 were derived from mouse chromosome 17. One of the chromosome 17-derived cosmid clones, 3-4-1 (located proximal to the T122/T66C segment) was found to be highly polymorphic among European wild-mouse populations and may be a useful probe to elucidate the evolution and migration of Mus species. The randomly isolated mouse-derived cosmid clones can also be screened for the presence of functional genes. Using testicular cDNA as a probe, a testis-specific gene was cloned from mouse chromosome 17. |
