| First Author | Chen C | Year | 1987 |
| Journal | Biochem Biophys Res Commun | Volume | 146 |
| Issue | 3 | Pages | 1311-7 |
| PubMed ID | 3304292 | Mgi Jnum | J:35014 |
| Mgi Id | MGI:82466 | Doi | 10.1016/0006-291x(87)90792-3 |
| Citation | Chen C, et al. (1987) Isolation of the Candida tropicalis gene for P450 lanosterol demethylase and its expression in Saccharomyces cerevisiae. Biochem Biophys Res Commun 146(3):1311-7 |
| abstractText | We have isolated the gene for cytochrome P450 lanosterol 14 alpha-demethylase (14DM) from the yeast Candida tropicalis. This was accomplished by screening genomic libraries of strain ATCC750 in E. coli using a DNA fragment containing the yeast Saccharomyces cerevisiae 14DM gene. Identity of this gene was confirmed by a) observing a heme binding region common to all P450s after sequencing the 3' portion of the gene, and b) based upon tests of its expression in strains of Saccharomyces cerevisiae. |
