| First Author | Lynch F | Year | 1989 |
| Journal | Eur J Immunol | Volume | 19 |
| Issue | 2 | Pages | 223-9 |
| PubMed ID | 2784763 | Mgi Jnum | J:26012 |
| Mgi Id | MGI:73802 | Doi | 10.1002/eji.1830190202 |
| Citation | Lynch F, et al. (1989) Mouse strain variation in Ly-24 (Pgp-1) expression by peripheral T cells and thymocytes: implications for T cell differentiation. Eur J Immunol 19(2):223-9 |
| abstractText | The cell surface glycoprotein Ly-24 has been proposed as a useful marker for the identification of in vivo-primed T cells. Analysis of Ly-24 surface expression by T cells from different mouse strains has shown variation in Ly-24 expression that is not H-2 linked; however, mice of the Ly-24.1 allele (e.g. BALB/c) express relatively high amounts, whereas Ly-24.2 strains (e.g. C57BL/6) are low expressors. In BALB/c (Ly-24 high) and C57BL/6 (Ly-24 low) mice, Ly-24 was expressed by both CD4- CD8+ and CD4+ CD8- subpopulations of single-positive T cells and thymocytes Among CD4- CD8- thymocytes, the overall expression of Ly-24 was similar in both mouse strains. Analysis of CD4+ and CD8+ single-positive thymocytes from newborn and adult BALB/c mice showed that the neonatal population contained fewer Ly-24+ cells. However, using the cell surface markers J11d and CD3, neonatal single-positive thymocytes were found to contain larger numbers of cells with the Ly-24-J11d+CD3 low to negative phenotype. Taken together, these results show that in BALB/c (Ly-24 high) mice, as soon as functional mature phenotype (CD3+) CD4+ and CD8+ single-positive thymocytes are generated, they already express Ly-24. These data cast doubt on the usefulness of Ly-24 expression as a universal marker of in vivo-primed T cells and suggest that in BALB/c mice thymus migrants may well be Ly-24+. Expression of Ly-24 by thymocytes is discussed in the context of current models of intrathymic T cell differentiation. |
