| First Author | Moura-Neto R | Year | 1989 |
| Journal | Proc Natl Acad Sci U S A | Volume | 86 |
| Issue | 11 | Pages | 3997-4001 |
| PubMed ID | 2726762 | Mgi Jnum | J:9816 |
| Mgi Id | MGI:58273 | Doi | 10.1073/pnas.86.11.3997 |
| Citation | Moura-Neto R, et al. (1989) An element downstream of the cap site is required for transcription of the gene encoding mouse ribosomal protein L32. Proc Natl Acad Sci U S A 86(11):3997-4001 |
| abstractText | To identify the elements that regulate transcription of the mouse gene encoding ribosomal protein L32 (rpL32), we transfected monkey kidney (COS or CV-1) cells with mutants bearing progressive 5' deletions or an internal deletion in exon I and measured their transient expression by S1 nuclease protection analysis. When the mutant genes were tested in the vector pi SVHSplac, which contains a short segment of the oriregion of simian virus 40, maximum expression was observed with as little as 36 base pairs of 5' flanking sequence, and the mutant bearing the exon I deletion was expressed very efficiently. However, when the genes were tested in a simple prokaryotic (pUC) vector, the expression was increased 3- to 4-fold by sequences between -36 and -159, and the exon I segment was absolutely required for expression. Gel mobility-shift and methylation interference analyses revealed that a nuclear factor specifically binds to a GGCTGCCATC sequence within this exon I segment. These results, taken together with other recent findings, indicate that the elements involved in transcriptional regulation of the rpL32 gene are distributed over a 200-base-pair region that spans the cap site. The contributions of some of these elements are apparently masked in the presence of simian virus 40 ori-region elements. |
