| First Author | Sanchez P | Year | 1990 |
| Journal | J Immunol | Volume | 144 |
| Issue | 7 | Pages | 2816-20 |
| PubMed ID | 2108215 | Mgi Jnum | J:10387 |
| Mgi Id | MGI:58839 | Doi | 10.4049/jimmunol.144.7.2816 |
| Citation | Sanchez P, et al. (1990) Mouse V lambda x gene sequence generates no junctional diversity and is conserved in mammalian species. J Immunol 144(7):2816-20 |
| abstractText | The lambda x, a new mouse Ig lambda L chain, is produced by rearrangement of the V lambda x, J lambda 2, and C lambda 2 gene segments. The V lambda x amino acid sequence is as divergent to other V lambda as to Vk gene sequences. Additionally, its third hypervariable region (CDR3) is four amino acids longer than those of all other variable gene segments of murine L chain. We have cloned and sequenced the germ-line V lambda x gene and found that the unexpected CDR3 length is encoded by the V lambda x gene. Junctional diversity is prevented by a TAA termination codon localized at the V lambda x 3' extremity. Moreover, we show a striking conservation of the V lambda x sequence in various mammalian species. Portions of the V lambda x sequence display more than 70% of nucleotide sequence identity with rabbit and human variable regions. These results suggest that V lambda x predated the divergence of mammalian species. |
