| First Author | Stubbs L | Year | 1990 |
| Journal | Genomics | Volume | 7 |
| Issue | 3 | Pages | 422-7 |
| PubMed ID | 1973141 | Mgi Jnum | J:10597 |
| Mgi Id | MGI:59045 | Doi | 10.1016/0888-7543(90)90177-v |
| Citation | Stubbs L, et al. (1990) The murine genes Hox-5.1 and Hox-4.1 belong to the same HOX complex on chromosome 2. Genomics 7(3):422-7 |
| abstractText | Two different loci of Antennapedia-related homeobox-containing genes have been shown to map to mouse chromosome 2: the HOX-5 complex and the Hox-4.1 gene. These independently derived loci are likely to be parts of a single gene complex, although their close linkage has not yet been demonstrated. Since cosmid walks to extend the HOX-5 cluster and to potentially link the two loci were unsuccessful, we have used large restriction fragments separated by pulsed-field gel electrophoresis to demonstrate the linkage between probes from the HOX-5 region and sequences near Hox-4.1. To further define the distance between the two linked loci, we screened a NotI jumping library with sequences near the Hox-5.1 gene to obtain a marker within the region predicted to contain Hox-4.1. The jumping endpoint lies within genomic clones from a lambda phage walk extending from the 5' end of Hox-4.1, and thus provides clear evidence of linkage between the two Hox loci. Our results demonstrate that Hox-4.1 lies approximately 35 kb downstream of the Hox-5.1 gene and that the two loci do indeed thus constitute parts of the same HOX complex. |
