| First Author | Gleditsch L | Year | 1990 |
| Journal | Immunogenetics | Volume | 32 |
| Issue | 5 | Pages | 297-303 |
| PubMed ID | 1979062 | Mgi Jnum | J:10868 |
| Mgi Id | MGI:59313 | Doi | 10.1007/BF00211642 |
| Citation | Gleditsch L, et al. (1990) No recombinations between Tcra-V and Tcra-C gene segments in 669 backcross mice. Immunogenetics 32(5):297-303 |
| abstractText | Because T-cell receptor (Tcr) genes may possibly function as non-major histocompatibility complex (MHC) immune response genes or predispose for autoimmune diseases, it is important to know how these genes are inherited. We found that Bgl I-digested DNA of BALB/c, C3H, DBA/2, and C57BL/6 exhibited restriction enzyme fragment length polymorphisms (RFLPs) for the Tcra-V1, Tcra-V2, Tcra-V4, Tcra-V6, Tcra-V7, Tcra-V8, Tcra-V11, Tcra-V12, Tcra-V13, and Tcra-C gene segments. Inheritance of these RFLPs in 669 offspring from (BALB/c x C57BL/6) x BALB/c, (BALB/c x C57BL/6) x C57BL/6, (C57BL/6 x DBA2) x DBA/2, and (C57BL/6 x C3H) x C3H backcrosses was studied. Since we did not find any recombinations in the offspring, Tcra-V and Tcra-C gene segments are tightly linked and inherited as a haplotype. A peculiar finding was that 22 out of 103 (BALB/c x C57BL/6) x BALB/c offspring, heterozygous for Tcra-C, had deleted a C57BL/6 Tcra-V1 band as well as Tcra-V2 and Tcra-V4 bands. As will be discussed, this deletion is probably caused by heterogeneity in the C57BL/6 breeding stock of a commercial supplier. In seven BXD and BXH recombinant inbred strains with known recombinations between the Tcra-C and Es-10 loci, all Tcra-V RFLPs cosegregated with the Tcra-C RFLP. This finding agrees with the conclusion from our backcross studies; namely that Tcra-V and Tcra-C gene segments are tightly linked. |
