| First Author | Broccoli D | Year | 1991 |
| Journal | Genomics | Volume | 10 |
| Issue | 1 | Pages | 68-74 |
| PubMed ID | 2045111 | Mgi Jnum | J:11242 |
| Mgi Id | MGI:59681 | Doi | 10.1016/0888-7543(91)90485-w |
| Citation | Broccoli D, et al. (1991) Isolation of a variant family of mouse minor satellite DNA that hybridizes preferentially to chromosome 4. Genomics 10(1):68-74 |
| abstractText | Two cosmids (HRS-1 and HRS-2) containing mouse minor satellite DNA sequences have been isolated from a mouse genomic library. In situ hybridization under moderate stringency conditions to metaphase chromosomes from RCS-5, a tumor cell line derived from the SJL strain, mapped both HRS-1 and HRS-2 to the centromeric region of chromosome 4. Sequence data indicate that these cloned minor satellite DNA sequences have a basic higher order repeat of 180 bp, composed of three diverged 60-bp monomers. Digestion of mouse genomic DNA with several restriction enzymes produces a ladder of minor satellite fragments based on a 120-bp repeat. The restriction enzyme NlaIII (CATG) digests all the minor satellite DNA into three prominent bands of 120, 240, and 360 bp and a weak band of 180 bp. Thus, the majority of minor satellite sequences in the genome are arranged in repeats based on a 120-bp dimer, while the family of minor satellite sequences described here represents a rare variant of these sequences. Our results raise the possibility that there may be other variant families of minor satellites analogous to those of alphoid DNA present in humans. |
