| First Author | Ching GY | Year | 1991 |
| Journal | J Biol Chem | Volume | 266 |
| Issue | 29 | Pages | 19459-68 |
| PubMed ID | 1717465 | Mgi Jnum | J:11497 |
| Mgi Id | MGI:59930 | Doi | 10.1016/s0021-9258(18)55018-3 |
| Citation | Ching GY, et al. (1991) Structure of the gene for the neuronal intermediate filament protein alpha-internexin and functional analysis of its promoter. J Biol Chem 266(29):19459-68 |
| abstractText | We have isolated the gene encoding the neuronal intermediate filament protein alpha-internexin using low stringency hybridization conditions and an NF-M (neurofilament middle molecular weight subunit) cDNA probe. This gene consists of three exons and two introns. The sequence data and the exon-intron organization of the gene establish its classification as a type IV intermediate filament gene. Transient transfection experiments showed that up to 5 kilobases of the alpha-internexin promoter region transcribed equally well in both the alpha-internexin-expressing and -nonexpressing cell lines. The results also demonstrated that the region from -77 to +73 relative to the transcription start site was sufficient for accurate basal transcription, but inclusion of the -254 to -78 region was required for efficient transcription. Sequence analysis shows that the -254 to -78 region contains several potential positive regulatory elements. |
