| First Author | Tait JF | Year | 1992 |
| Journal | Genomics | Volume | 12 |
| Issue | 2 | Pages | 313-8 |
| PubMed ID | 1346776 | Mgi Jnum | J:12689 |
| Mgi Id | MGI:60924 | Doi | 10.1016/0888-7543(92)90379-7 |
| Citation | Tait JF, et al. (1992) Chromosomal mapping of the human annexin IV (ANX4) gene. Genomics 12(2):313-8 |
| abstractText | Annexin IV (placental anticoagulant protein II) is a member of the annexin or lipocortin family of calcium-dependent phospholipid-binding proteins. A cDNA for human annexin IV was isolated from a placental library that is 675 bases longer in the 3' untranslated region than previously reported, indicating the existence of alternative mRNA processing for this gene. Genomic Southern blotting with a cDNA probe indicated a gene size of 18-56 kb. Primers developed for polymerase chain reaction (PCR) allowed amplification of a 1.6-kb portion of the ANX4 gene. DNA sequence analysis showed that this PCR product contained a single intron with exon-intron boundaries in exactly the same position as in the mouse annexin I and annexin II genes. PCR analysis of a somatic cell hybrid panel mapped the ANX4 gene to chromosome 2, and in situ hybridization with a cDNA probe showed a unique locus for ANX4 at 2p13. This study provides further evidence that genes for the annexins are dispersed throughout the genome but are similar in size and exon-intron organization. |
