| First Author | Cobb RR | Year | 1992 |
| Journal | Genomics | Volume | 12 |
| Issue | 2 | Pages | 405-8 |
| PubMed ID | 1346782 | Mgi Jnum | J:1789 |
| Mgi Id | MGI:50313 | Doi | 10.1016/0888-7543(92)90393-7 |
| Citation | Cobb RR, et al. (1992) A malic enzyme probe detects cross-hybridizing sequences closely linked to loci encoding other metabolic enzymes. Genomics 12(2):405-8 |
| abstractText | The cytoplasmic malic enzyme (Mod-1) catalyzes the oxidative decarboxylation of malate: malate + NADP+----pyruvate + CO2 + NADPH + H+. Using a cDNA clone of Mod-1 as a probe, two new DNA markers not at the Mod-1 locus (restriction fragment length polymorphisms, RFLP) were detected by Southern blot analysis that showed extensive homology to Mod-1 sequences. Linkage of each restriction fragment length polymorphism to loci other than Mod-1 was assessed using the BXD (C57BL/6J x DBA/2J) recombinant inbred strains and confirmed by backcrosses. One polymorphic site, designated D9Rti1, was found to be closely linked to the phosphoglucomutase (Pgm-3) locus on Chromosome 9. The other hybridization site, designated D1Rti2, was closely linked to the isocitrate dehydrogenase (Idh-1) locus on Chromosome 1. The data presented imply that Mod-1 homologous sequences are tightly linked to three different metabolic enzymes. |
